RBS

Part:BBa_K4017002:Design

Designed by: César Daniel Marques Duarte   Group: iGEM21_NOVA_LxPortugal   (2021-09-30)


Ribosome Binding Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part encodes for the binding region of bacterial ribosomes in mRNA and ensures the translation of both the signal peptides used by the team (BBa_K4017003 - BBa_K4017007) and the proteins for plastic degradation PETase and MHETase (BBa_K4017010 - BBa_K4017013). The Ribosome Binding Site (RBS) is a rich A/G region, upstream of the start codon ATG of an mRNA. Translation initiation in bacteria requires RBS, as it is responsible for the recruitment of the small bacterial ribosome (30S) and the translation initiation [1-2].

Source

Adapted from the genomic sequence of Escherichia coli DH5α.

References

[1]: Hu, Yangbo, et al. "Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions." PloS one 7.11 (2012): e50142.

[2]: Omotajo, Damilola, et al. "Distribution and diversity of ribosome binding sites in prokaryotic genomes." BMC genomics 16.1 (2015): 1-8.